eDNA
Introduction
The EDNA platform specializes in the metabarcoding approach to identify microbial, animal and plant communities using environmental samples (soil, water, feces, etc.). Usually, DNA metabarcode is designed according to the scientific question, and targets multi-copy DNA (chloroplast DNA, mitochondrial DNA, nuclear ribosomal DNA). The eDNA platform can provide support for:
- the design of experiments according to the scientific question, technical constraints and strategy to ensure data quality;
- large-scale DNA extraction;
- large-scale DNA amplification;
- the outsourcing of sequencing with a company that provides a high quality service for amplicon sequencing;
- the analysis of the raw sequences towards the production of a rating table that will later be analyzed by the ecologist;
- the discussion on scientific interpretation according to the potential pitfalls of the metabarcoding approach.
Proposed services
- Analytical services
Detailed service offer
- Design of experiments according to the scientific question, technical constraints and strategy to ensure data quality;
- Large-scale DNA extraction;
- Large-scale DNA amplification;
- Outsourcing of sequencing with a company that provides a high quality service for amplicon sequencing;
- Analysis of the raw sequences towards the production of a rating table that will later be analyzed by the ecologist;
- Scientific expertise for interpretation according to the potential pitfalls of the metabarcoding approach.
Analytical platforms
Stephen Mulero
Technical manager
Contact by email
Location
ECA, UMR UGA-USMB-CNRS 5553 Université Grenoble Alpes CS 40700, 38058 Grenoble cedex 9, France
