Tandem MS imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra
Résumé
Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this report, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray paren-chyma cells but also in certain oil cells and, further,...
Auteurs, date et publication :
Auteurs Tingting Fu , David Touboul , Serge Della-Negra , Emeline Houël , Nadine Amusant , Christophe Duplais , Gregory L Fisher , Alain Brunelle
Publication : Analytical Chemistry
Date : 2018
Pages : acs.analchem.8b01157