Auteurs, date et publication :
Auteurs Marisa de Campos-Santana , Mario Amalfi , Gabriel Castillo , Cony Decock
Publication : Mycologia
Date : 2025
Volume : 108
Issue : 5
Pages : 939-953
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs Bibiana Rojas , Emily Burdfield-Steel , Chiara De Pasqual , Swanne Gordon , Linda Hernández , Johanna Mappes , Ossi Nokelainen , Katja Rönkä , Carita Lindstedt
Publication : Frontiers in Ecology and Evolution
Date : 2025
Volume : 6
Pages : 93
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs Clémentine Renneville , Arnaud Le Rouzic , Michel Baylac , Alexis Millot , Stéphane Loisel , Eric Edeline
Publication : Oikos
Date : 2025
Volume : 125
Issue : 8
Pages : 1193-1202
Catégorie(s)
#CNRS #ENS #PLANAQUARésumé
Abstract Macroinvertebrate assemblages are the most common bioindicators used for stream biomonitoring, yet the standard approach exhibits several time-consuming steps, including the sorting and identification of organisms based on morphological criteria. In this study, we examined if DNA metabarcoding could be used as an efficient molecular-based alternative to the morphology-based monitoring of streams using macroinvertebrates. We compared results achieved with the standard morphological identification of organisms sampled in 18 sites located on 15 French wadeable streams to results obtained with the DNA metabarcoding identification of sorted bulk material of the same macroinvertebrate samples, using read numbers (expressed as relative frequencies) as a proxy for abundances. In particular, we evaluated how combining and filtering metabarcoding data obtained from three different markers (COI: BF1-BR2, 18S: Euka02 and 16S: Inse01) could improve the efficiency of bioassessment. In total, 140 taxa were identified based on morphological criteria, and 127 were identified based on DNA metabarcoding using the three markers, with an overlap of 99 taxa. The threshold values used for sequence filtering based on the “best identity” criterion and the number of reads had an effect on the assessment efficiency of data obtained with each marker. Compared to single marker results, combining data from different markers allowed us to improve the match between biotic index values obtained with the bulk DNA versus morphology-based approaches. Both approaches assigned the same ecological quality class to a majority (86%) of the site sampling events, highlighting both the efficiency of metabarcoding as a biomonitoring tool but also the need for further research to improve this efficiency.
Auteurs, date et publication :
Auteurs Albin Meyer , Frédéric Boyer , Alice Valentini , Aurélie Bonin , Gentile Francesco Ficetola , Jean-Nicolas Beisel , Jonathan Bouquerel , Philippe Wagner , Coline Gaboriaud , Florian Leese , Tony Dejean , Pierre Taberlet , Philippe Usseglio-Polatera
Publication : Molecular Ecology
Date : 2025
Volume : 30
Issue : 13
Pages : 3203–3220
Catégorie(s)
#CNRS #eDNARésumé
Our knowledge of the factors influencing the distribution of soil organisms is limited to specific taxonomic groups. Consequently, our understanding of the drivers shaping the entire soil multitrophic network is constrained. To address this gap, we conducted an extensive soil biodiversity monitoring program in the French Alps, using environmental DNA to obtain multi-taxon data from 418 soil samples. The spatial structure of resulting soil multitrophic networks varied significantly between and within habitats. From forests to grasslands, we observed a shift in the abundance of trophic groups from fungal to bacterial feeding channels, reflecting different ecosystem functioning. Furthermore, forest soil networks were more strongly spatially structured which could only partly be explained by abiotic conditions. Grassland soil networks were more strongly driven by plant community composition and soil characteristics. Our findings provide valuable insights into how climate and land-use changes may differentially affect soil multitrophic networks in mountains.
Auteurs, date et publication :
Auteurs Irene Calderon-Sanou , Marc Ohlmann , Tamara Munkemuller , Lucie Zinger , Mickael Hedde , Clement Lionnet , Camille Martinez-Almoyna , Amelie Saillard , Julien Renaud , Nicolas Le Guillarme , Ludovic Gielly , Wilfried Thuiller , Orchamp Consortium
Publication : SOIL BIOLOGY & BIOCHEMISTRY
Date : 2024
Volume : 190
Catégorie(s)
#ANR-Citation #CNRS #Lautaret #UGAAuteurs, date et publication :
Auteurs François Feer , Jean-François Ponge , Sylvie Jouard , Doris Gomez
Publication : Ecological Research
Date : 2025
Volume : 28
Issue : 1
Pages : 93-102
Catégorie(s)
#CNRS #FORET NouraguesRésumé
Monoterpenes (MTs) represent an important family of biogenic volatile organic compounds (BVOCs) in terms of amount and chemical diversity. This family has been extensively studied using gas chromatography (GC) and proton transfer reaction-mass spectrometry (PTR-MS). Upon recent advances with Fast Gas Chromatography (FastGC), it was also commercialized with proton transfer reaction-time of flight-mass spectrometry (PTR-ToF-MS) instruments. The combination of both techniques showed promising results in the near real-time separation of isomers, with the need of further improvements. In this study, a FastGC prototype was coupled to a conventional PTR-MS (PTR-QuadMS). Extensive laboratory experiments were performed, in order to test the system’s performance and to optimize its operational parameters for MT separation. The detection limit was determined to be around 0.8–1.7 ppbv, depending on the MT. The system was afterwards deployed during a three-week field campaign in a mixed holm oak (Quercus ilex) forest known for its important MT emissions. MTs were measured in the incoming and the outgoing air of dynamic enclosures installed on the branches of four different trees. Three chemotypes of holm oak trees could be distinguished showing consistently different proportions of the emitted MTs throughout the measurement campaign: pinene-type, myrcene-type and limonene-type. Measurements showed a systematic diel variation in emissions typical of light and temperature-dependent, de novo-synthesized VOCs. The results demonstrated the feasibility of the FastGC/PTR-MS system for continuous measurements from dynamic chambers in the field, whereas further improvements would be necessary to lower the detection limit for ambient air measurements.
Auteurs, date et publication :
Auteurs Sandy Bsaibes , Felix Piel , Valérie Gros , François Truong , Florence Lafouge , Raluca Ciuraru , Pauline Buysse , Julien Kammer , Benjamin Loubet , Michael Staudt
Publication : Atmosphere
Date : 2020
Volume : 11
Issue : 7
Pages : 690
Catégorie(s)
#CNRS #FORET Puechabon #INRAE #PT-RMSAuteurs, date et publication :
Auteurs Cristiano S Costa , Gonzalo Giribet FLS , Ricardo Pinto-Da-Rocha
Publication : Zoological Journal of the Linnean Society
Date : 2025
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs Jean-Marc Limousin , Serge Rambal , Jean-Marc Ourcival , Jesus Rodrıguez-Calcerrada , Ignacio M Perez-Ramos , Raquel Rodrıguez-Cortina , Laurent Misson , Richard Joffre
Publication : Oecologia
Date : 2025
Volume : 169
Pages : 565-577
Catégorie(s)
#CNRS #FORET PuechabonAuteurs, date et publication :
Auteurs D. Audigeos , L. Brousseau , S. Traissac , C. Scotti-Saintagne , I. Scotti
Publication : Journal of Evolutionary Biology
Date : 2013
Volume : 26
Issue : 3
Pages : 529–544