Auteurs, date et publication :

Auteurs Mario Amalfi , Gerardo Robledo , Cony Decock

Publication : Mycological progress

Date : 2025

Volume : 13

Issue : 4

Pages : 995


Catégorie(s)

#CNRS #FORET Nouragues

Auteurs, date et publication :

Auteurs Mario Amalfi , Cony Antonio Decock

Publication : Mycologia

Date : 2025


Catégorie(s)

#CNRS #FORET Nouragues

Résumé

Dietary methionine affects protein metabolism, lean gain and growth performance and acts in the control of oxidative stress. When supplied in large excess relative to growth requirements in diets for pigs, positive effects on pork quality traits have been recently reported. This study aimed to decipher the molecular and biochemical mechanisms affected by a dietary methionine supply above growth requirements in the loin muscle of finishing pigs. During the last 14 days before slaughter, crossbred female pigs (n = 15 pigs/diet) were fed a diet supplemented with hydroxy-methionine (Met5; 1.1% of methionine) or not (CONT, 0.22% of methionine). Blood was sampled at slaughter to assess key metabolites. At the same time, free amino acid concentrations and expression or activity levels of genes involved in protein or energy metabolism were measured in the longissimus lumborum muscle (LM). The Met5 pigs exhibited a greater activity of creatine kinase in plasma when compared with CONT pigs. The concentrations of free methionine, alpha-aminobutyric acid, anserine, 3-methyl-histidine, lysine, and proline were greater in the LM of Met5 pigs than in CONT pigs. Expression levels of genes involved in protein synthesis, protein breakdown or autophagy were only scarcely affected by the diet. Among ubiquitin ligases, MURF1, a gene known to target creatine kinase and muscle contractile proteins, and OTUD1 coding for a deubiquitinase protease, were up-regulated in the LM of Met5 pigs. A lower activity of citrate synthase, a reduced expression level of ME1 acting in lipogenesis but a higher expression of PPARD regulating energy metabolism, were also observed in the LM of Met5 pigs compared with CONT pigs. Principal component analysis revealed that expression levels of many studied genes involved in protein and energy metabolism were correlated with meat quality traits across dietary treatments, suggesting that subtle modifications in expression of those genes had cumulative effects on the regulation of processes leading to the muscle transformation into meat. In conclusion, dietary methionine supplementation beyond nutritional requirements in pigs during the last days before slaughter modified the free amino acid profile in muscle and its redox capacities, and slightly affected molecular pathways related to protein breakdown and energy metabolism. These modifications were associated with benefits on pork quality traits.


Auteurs, date et publication :

Auteurs Florence Gondret , Nathalie Floc'h , Dolores I. Batonon-Alavo , Marie-Hélène Perruchot , Yves Mercier , Bénédicte Lebret

Publication : Animal

Date : 2021

Volume : 15

Pages : 100268


Catégorie(s)

#CNRS #EcoGenO #Université de Rennes

Auteurs, date et publication :

Auteurs Eva Ringler , Andrius Pašukonis , W Tecumseh Fitch , Ludwig Huber , Walter Hödl , Max Ringler

Publication : Behavioral Ecology

Date : 2025

Volume : 26

Issue : 4

Pages : 1219-1225


Catégorie(s)

#CNRS #FORET Nouragues

Résumé

This paper assesses the options available to researchers analysing multilevel (including longitudinal) data, with the aim of supporting good methodological decision-making. Given the confusion in the literature about the key properties of fixed and random effects (FE and RE) models, we present these models’ capabilities and limitations. We also discuss the within-between RE model, sometimes misleadingly labelled a ‘hybrid’ model, showing that it is the most general of the three, with all the strengths of the other two. As such, and because it allows for important extensions—notably random slopes—we argue it should be used (as a starting point at least) in all multilevel analyses. We develop the argument through simulations, evaluating how these models cope with some likely mis-specifications. These simulations reveal that (1) failing to include random slopes can generate anti-conservative standard errors, and (2) assuming random intercepts are Normally distributed, when they are not, introduces only modest biases. These results strengthen the case for the use of, and need for, these models.


Auteurs, date et publication :

Auteurs Andrew Bell , Malcolm Fairbrother , Kelvyn Jones

Publication : Quality & Quantity

Date : 2019

Volume : 53

Issue : 2

Pages : 1051-1074


Catégorie(s)

#CNRS #ENS

Résumé

Advancing the field of ecology relies on understanding generalities and developing theories based on empirical and functional relationships that integrate across organismal to global spatial scales and span temporal scales. Significant advances in predicting responses of ecological communities to globally extensive anthropogenic perturbations, for example, require understanding the role of environmental context in determining outcomes, which in turn requires standardized experiments across sites and regions. Distributed collaborative experiments can lead to high-impact advances that would otherwise be unachievable. Here, we provide specific advice and considerations relevant to researchers interested in employing this emerging approach using as a case study our experience developing and running the Nutrient Network, a globally distributed experimental network (currently >75 sites in 17 countries) that arose from a grassroots, cooperative research effort. We clarify the design, goals and function of the Nutrient Network as a model to empower others in the scientific community to employ distributed experiments to advance our predictive understanding of global-scale ecological trends and responses. Our experiences to date demonstrate that globally distributed experimental science need not be prohibitively expensive or time-consuming on a per capita basis and is not limited to senior scientists or countries where science is well funded. While distributed experiments are not a panacea for understanding ecological systems, they can substantially complement existing approaches.


Auteurs, date et publication :

Auteurs Elizabeth T. Borer , W. Stanley Harpole , Peter B. Adler , Eric M. Lind , John L. Orrock , Eric W. Seabloom , Melinda D. Smith

Publication : Methods in Ecology and Evolution

Date : 2025

Volume : 5

Issue : 1

Pages : 65-73


Catégorie(s)

#CEREEP #CNRS #ENS

Auteurs, date et publication :

Auteurs Weslly Franco , Natalia Ladino , Jacques HC Delabie , Alain Dejean , Jérôme Orivel , Mélanie Fichaux , Sarah Groc , Maurice Leponce , Rodrigo M Feitosa

Publication : Zootaxa

Date : 2025

Volume : 4674

Issue : 5

Pages : 509-543


Catégorie(s)

#CNRS #FORET Nouragues

Résumé

This paper presents the first record of Chomelia triflora from Brazil, to date a species only known from French Guiana. After examining herbaria collections and doing fieldwork in the Brazilian Amazon, we found that the species also occurs in and around the Ducke Reserve in the state of Amazonas, Brazil. Our finding increases the data about the Brazilian Amazon and contributes to the better knowledge of Chomelia in Brazil.


Auteurs, date et publication :

Auteurs Maria do Céo R. Pessoa , Claes Persson , Alexandre Antonelli , Maria Regina de V. Barbosa

Publication : Check List

Date : 2017

Volume : 13

Issue : 4

Pages : 159-162


Catégorie(s)

#CNRS #FORET Nouragues

Auteurs, date et publication :

Auteurs R Pessoa Maria do Céo , Claes Persson , Alexandre Antonelli , Maria Regina de V Barbosa

Publication : Check List

Date : 2023

Volume : 13

Pages : 159


Catégorie(s)

#CNRS #FORET Nouragues

Auteurs, date et publication :

Auteurs José Teston , Margarida Freitas

Publication : Check List

Date : 2025

Volume : 11

Pages : 1


Catégorie(s)

#CNRS #FORET Nouragues