Résumé
Subalpine grassland ecosystems are important from biodiversity, agriculture, and touristic perspectives but their resilience to seasonally occurring climatic extremes is increasingly challenged with climate change, accelerating their vulnerability to tipping points. Microbial communities, which are central in ecosystem functioning, are usually considered as more resistant and highly resilient to such extreme events due to their functional redundancy and strong selection in residing habitats. To investigate this, we explored soil microbial responses upon recurrent summer droughts associated with early snowmelt in subalpine grasslands mesocosms set-up at the Lautaret Pass (French Alps). Potential respiration, nitrification and denitrification were monitored over a period of two growing seasons along with quantification of community gene abundances of total bacteria as well as (de)nitrifiers. Results revealed that droughts had a low and short-term adverse impact on bacterial total respiration supporting their hypothesized high resilience, i.e., resistance and ability to recover. Nitrification and abundances of the corresponding functional guilds showed relatively strong resistance to summer droughts but declined in response to early snowmelt. This triggered a cascading effect on denitrification but also on abundances of denitrifying communities which recovered from all climatic extremes except from the summer droughts where nitrifiers were collapsed. Denitrification and respective functional groups faced high impact of applied stresses with strong reduction in abundance and activity of this specialized community. Although, consequently lower microbial competition for nitrate may be positive for plant biomass production, warnings exist when considering the potential nitrogen leaching from these ecosystems as well as risks of greenhouses gases emission such as N2O.
Auteurs, date et publication :
Auteurs Farhan Hafeez , Lionel Bernard , Franck Poly , Jean-Christophe Clément , Thomas Pommier
Date : 2021
Pages : 2021.03.15.435477
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Rock glaciers, usually preserved in periglacial environments of cold-climate high-relief regions, are useful (palaeo)climate indicators. In the European Alps, numerous studies have focused on describing these geomorphological features. However, very few have attempted directly dating them. This lack of measurement is particularly evident in the French Alps. To fill this gap, we performed Cosmic Ray Exposure (CRE) dating through beryllium-10 (10Be) on 10 granitic rock samples collected from two relict rock glaciers, hereafter referred as RG1 and RG2, located at col du Lautaret in the French Alps. This first CRE dating of rock glaciers in the French Alps yielded mean ages of 11.8 ± 0.5 kyrs and 11.6 ± 0.6 kyrs for RG1 and RG2, respectively. While RG2 is clearly a talus-derived rock glacier, the origin of RG1 is less clear. It could potentially be talus-derived, glacier-derived or of mixed origin. The 10Be ages indicate that both rock glaciers became inactive during the transition between the Younger Dryas and the onset of the Holocene, consistently with other studies in the Northern Hemisphere. Considering surface velocity measurements carried out since the 1980s on neighbouring rock glaciers, we hypothesise that the rock glaciers were formed either during the Younger Dryas or slightly earlier, between the Heinrich Stadial 1 and the Younger Dryas. During this period, we estimated temperatures ~3.6 °C lower than the 1980s and precipitation up to 30% lower than at present.
Auteurs, date et publication :
Auteurs Joanna Charton , Deborah Verfaillie , Vincent Jomelli , Bernard Francou
Publication : Geomorphology
Date : 2025
Volume : 394
Pages : 107962
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Alpine treelines globally may move upslope due to climatic warming. Such movement would need, as the first steps, seed germination and seedling establishment ab
Auteurs, date et publication :
Auteurs Hannah Loranger , Gerhard Zotz , Maaike Y. Bader
Publication : AoB PLANTS
Date : 2016
Volume : 8
Catégorie(s)
#CNRS #Lautaret #UGARésumé
In the French Alps, Soldanella alpina (S. alpina) grow under shade and sun conditions during the vegetation period. This species was investigated as a model for the dynamic acclimation of shade leaves to the sun under natural alpine conditions, in terms of photosynthesis and leaf anatomy. Photosynthetic activity in sun leaves was only slightly higher than in shade leaves. The leaf thickness, the stomatal density and the epidermal flavonoid content were markedly higher, and the chlorophyll/flavonoid ratio was significantly lower in sun than in shade leaves. Sun leaves also had a more oxidised plastoquinone pool, their PSII efficiency in light was higher and their non-photochemical quenching (NPQ) capacity was higher than that of shade leaves. Shade-sun transferred leaves increased their leaf thickness, stomatal density and epidermal flavonoid content, while their photosynthetic activity and chlorophyll/flavonoid ratio declined compared to shade leaves. Parameters indicating protection against high light and oxidative stress, such as NPQ and ascorbate peroxidase, increased in shade-sun transferred leaves and leaf mortality increased. We conclude that the dynamic acclimation of S. alpina leaves to high light under alpine conditions mainly concerns anatomical features and epidermal flavonoid acclimation, as well as an increase in antioxidative protection. However, this increase is not large enough to prevent damage under stress conditions and to replace damaged leaves.
Auteurs, date et publication :
Auteurs Anne-Claire Talhouët , Sylvie Meyer , Xavier Baudin , Peter Streb
Publication : Physiologia Plantarum
Date : 2025
Volume : 168
Issue : 3
Pages : 563-575
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Phenolic compounds play important ecological roles in alpine plants such as offering efficient UV protection at high level of incident sunlight. Methods to study those compounds are limited, as they require sophisticated analytical tools and are time-consuming. An alternative and portable device—Dualex©—has been recently developed to estimate the plant epidermal flavonoids by fluorescence sensing. Here, we assessed if the Dualex device accurately estimates the phenolic contents of three alpine plant species along an elevational gradient and be an alternative to the commonly used chemical methods. We characterized their leaf epidermal absorbance measured by the Dualex device, total phenol content assessed by the Folin–Ciocalteu assay and total flavonoid content estimated by high-performance liquid chromatography. The results showed that leaf epidermal absorbance was slightly positively correlated to total phenols for Rhododendron ferrugineum and Dryas octopetala, but not for Vaccinium myrtillus and to total flavonoids for the first species, but not for the two others. The leaf epidermal absorbance estimated by the Dualex device is not an accurate and universal predictor of total phenols or total flavonoid contents for alpine plant species. The limitations of this optical method could be mainly explained by the high intraspecific variability of plant chemical composition in heterogeneous environmental conditions met in alpine areas. We thus recommend a cautious use of this device to avoid misinterpretations.
Auteurs, date et publication :
Auteurs Tiphaine Lefebvre , Annie Millery-Vigues , Christiane Gallet
Publication : Alpine Botany
Date : 2016
Volume : 126
Issue : 2
Pages : 177-185
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Foundation plants shape the composition of local biotic communities and abiotic environments, but the impact of a plant’s intraspecific variations on these processes is poorly understood. We examined these links in the alpine cushion moss campion (Silene acaulis) on two neighboring mountain ranges in the French Alps. Genotyping of cushion plants revealed two genetic clusters matching known subspecies. The exscapa subspecies was found on both limestone and granite, while the longiscapa one was only found on limestone. Even on similar limestone bedrock, cushion soils from the two S. acaulis subspecies deeply differed in their impact on soil abiotic conditions. They further strikingly differed from each other and from the surrounding bare soils in fungal community composition. Plant genotype variations accounted for a large part of the fungal composition variability in cushion soils, even when considering geography or soil chemistry, and particularly for the dominant molecular operational taxonomic units (MOTUs). Both saprophytic and biotrophic fungal taxa were related to the MOTUs recurrently associated with a single plant genetic cluster. Moreover, the putative phytopathogens were abundant, and within the same genus (Cladosporium) or species (Pyrenopeziza brassicae), MOTUs showing specificity for each plant subspecies were found. Our study highlights the combined influences of bedrock and plant genotype on fungal recruitment into cushion soils and suggests the coexistence of two mechanisms, an indirect selection resulting from the colonization of an engineered soil by free‐living saprobes and a direct selection resulting from direct plant–fungi interactions.
Auteurs, date et publication :
Auteurs Julien Roy , Jean-Marc Bonneville , Patrick Saccone , Sébastian Ibanez , Cécile H. Albert , Marti Boleda , Maya Gueguen , Marc Ohlmann , Delphine Rioux , Jean-Christophe Clément , Sébastien Lavergne , Roberto A. Geremia
Publication : Ecology and Evolution
Date : 2025
Volume : 8
Issue : 23
Pages : 11568-11581
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Mycorrhizal fungi or endphytes colonize plant roots and their occurrence and composition depend on biotic and abiotic characteristics of the ecosystem. We investigated the composition of these microbial communities associated with Festuca paniculata, a slow growing species, which dramatically impacts functional plant diversity and the recycling of organic matter in subalpine grasslands. F. paniculata individuals from both mown and unmown grasslands were randomly collected and the microscopic observation of the plant roots revealed a difference in fungal colonization according to management. The ITS regions of root-associated fungi were amplified, cloned and sequenced. Bioinformatic analysis revealed a total of 43 and 35 phylotypes in mown and unmown grasslands respectively, highlighting a remarkable difference in the composition between both fungal communities. The phylotypes were assigned to 9 classes in which two classes Eurotiomycetes and Lecanoromycetes were specific to mown grasslands, while Tremellomycetes were specific to unmown grasslands and only five phylotypes were common to both locations. The comparative analysis of fungal lifestyles indicated the dominance of saprobes and a large proportion of endophytes compared to the mycorrhizal fungi (7/1 and 11/2 phylotypes in mown and unmown grasslands, respectively). Endophyte richness was greater in the unmown gassland than in the mown grassland and their relative proportion was twice higher. Our results suggest that endophytes may offer potential resources to F. paniculata and play an important role in the regulation of plant diversity.
Auteurs, date et publication :
Auteurs Bello Mouhamadou , Claire Molitor , Florence Baptist , Lucile Sage , Jean-Christophe Clément , Sandra Lavorel , Armelle Monier , Roberto A. Geremia
Publication : Fungal Diversity
Date : 2011
Volume : 47
Issue : 1
Pages : 55-63
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Abstract. 3-hydroxy fatty acids (3-OH FAs) with 10 to 18 C atoms are membrane lipids mainly produced by Gram-negative bacteria. They have been recently proposed as temperature and pH proxies in terrestrial settings. Nevertheless, the existing correlations between pH/temperature and indices derived from 3-OH FA distribution (RIAN, RAN15 and RAN17) are based on a small soil dataset (ca. 70 samples) and only applicable regionally. The aim of this study was to investigate the applicability of 3-OH FAs as mean annual air temperature (MAAT) and pH proxies at the global level. This was achieved using an extended soil dataset of 168 topsoils distributed worldwide, covering a wide range of temperatures (5 °C to 30 °C) and pH (3 to 8). The response of 3-OH FAs to temperature and pH was compared to that of established branched GDGT-based proxies (MBT'5Me/CBT). Strong linear relationships between 3-OH FA-derived indices (RAN15, RAN17 and RIAN) and MAAT/pH could only be obtained locally, for some of the individual transects. This suggests that these indices cannot be used as paleoproxies at the global scale using simple linear regression models, in contrast with the MBT'5Me and CBT. However, strong global correlations between 3-OH FA relative abundances and MAAT/pH were shown by using other algorithms (multiple linear regression, k-NN and random forest models). The applicability of the k-NN and random forest models for paleotemperature reconstruction was tested and compared with the MAAT record from a Chinese speleothem. The calibration based on the random forest model appeared to be the most robust. It showed similar trends with previously available records and highlighted known climatic events poorly visible when using local 3-OH FA calibrations. Altogether, these results demonstrate the potential of 3-OH FAs as paleoproxies in terrestrial settings.
Auteurs, date et publication :
Auteurs Pierre Véquaud , Sylvie Derenne , Alexandre Thibault , Christelle Anquetil , Giuliano Bonanomi , Sylvie Collin , Sergio Contreras , Andrew Nottingham , Pierre Sabatier , Norma Salinas , Wesley Philip Scott , Josef P. Werne , Arnaud Huguet
Publication : Biogeosciences Discussions
Date : 2020
Pages : 1-40
Catégorie(s)
#CNRS #Lautaret #UGAAuteurs, date et publication :
Auteurs N. Legay , C. Baxendale , K. Grigulis , U. Krainer , E. Kastl , M. Schloter , R. D. Bardgett , C. Arnoldi , M. Bahn , M. Dumont , F. Poly , T. Pommier , J. C. Clément , S. Lavorel
Publication : Annals of Botany
Date : 2014
Volume : 114
Issue : 5
Pages : 1011-1021
Catégorie(s)
#CNRS #Lautaret #UGARésumé
Despite considerable efforts devoted to investigate the community assembly processes driving plant invasions, few general conclusions have been drawn so far. Three main processes, generally acting as successive filters, are thought to be of prime importance. The invader has to disperse (1st filter) into a suitable environment (2nd filter) and succeed in establishing in recipient communities through competitive interactions (3rd filter) using two strategies: competition avoidance by the use of different resources (resource opportunity), or competitive exclusion of native species. Surprisingly, despite the general consensus on the importance of investigating these three processes and their interplay, they are usually studied independently. Here we aim to analyse these three filters together, by including them all: abiotic environment, dispersal and biotic interactions, into models of invasive species distributions. We first propose a suite of indices (based on species functional dissimilarities) supposed to reflect the two competitive strategies (resource opportunity and competition exclusion). Then, we use a set of generalised linear models to explain the distribution of seven herbaceous invaders in natural communities (using a large vegetation database for the French Alps containing 5,000 community-plots). Finally, we measure the relative importance of competitive interaction indices, identify the type of coexistence mechanism involved and how this varies along environmental gradients. Adding competition indices significantly improved model’s performance, but neither resource opportunity nor competitive exclusion were common strategies among the seven species. Overall, we show that combining environmental, dispersal and biotic information to model invasions has excellent potential for improving our understanding of invader success.
Auteurs, date et publication :
Auteurs Laure Gallien , Florent Mazel , Sébastien Lavergne , Julien Renaud , Rolland Douzet , Wilfried Thuiller
Publication : Biological Invasions
Date : 2015
Volume : 17
Issue : 5
Pages : 1407-1423