Résumé
Using organic waste products (OWP) in agriculture makes it possible to increase productivity with less use of mineral fertilizers. However, the lasting effect on soil microbial communities of an OWP application repeated over several years needs further investigation. In the present study, soils were sampled from two long-term field experiments: QualiAgro and Colmar (France), where different types of OWP characterized by more or less stable organic matter had been applied for more than 10 years, and were compared to a control treatment. At QualiAgro, the carbon inputs due to OWP application were greater (∼4 t C ha−1 every two years) than at Colmar (∼1.7 t C ha−1 every two years). On both sites, soil samples were taken more than six months after the last OWP input. At QualiAgro, soil organic carbon, N and P2O5 concentrations, pH, and CEC were increased by repeated OWP inputs, as compared to the control. Soil microbial community parameters were also lastingly affected by OWP application. A 50% increase in microbial biomass was observed with OWP with the most stable organic matter contents. The prokaryotic community structure was influenced: directly by the OWP applied, and indirectly by soil properties changes. Soil pH appeared as a major driver for structure of the soil prokaryotic community. Fungal community structure was only directly influenced by the OWP applied. Contrastingly, at Colmar, OWP application had no impact on soil chemical characteristics or microbial communities’ parameters. This was probably due to the smaller amount of OWP applied than at QualiAgro, and/or a longer delay between the OWP application and soil sampling. Altogether, our results show that, depending on its type, the applied OWP could produce a lasting increase in soil microbial biomass and shape microbial community structure.
Auteurs, date et publication :
Auteurs S. Sadet-Bourgeteau , S. Houot , S. Dequiedt , V. Nowak , V. Tardy , S. Terrat , D. Montenach , V. Mercier , B. Karimi , N. Chemidlin Prévost-Bouré , P. A. Maron
Publication : Applied Soil Ecology
Date : 2018
Volume : 125
Pages : 278-287
Catégorie(s)
#ANR-Citation #Genosol #INRAE #PROAuteurs, date et publication :
Auteurs Daniel Mika-Nsimbi Poultney , Mathias Christina , Antoine Versini
Publication : Plant and Soil
Date : 2025
Volume : 453
Issue : 1-2
Pages : 597-613
Catégorie(s)
#CIRAD #INRAE #PRO #PRO ReunionAuteurs, date et publication :
Auteurs Marie Eden , Horst H. Gerke , Sabine Houot
Publication : Agronomy for Sustainable Development
Date : 2025
Volume : 37
Issue : 2
Catégorie(s)
#INRAE #PRO #PRO QualiAgroRésumé
We investigated whether perennial soil organic amendments of pig slurry (PS) and methanized pig slurry (MPS) affect active bacterial communities and change the diversity and the C-flux of Volatile Organic Compounds (VOCs) emitted by soils compared to control plots without any fertilization (C). The long term effects of the fertilization history of the amendments and the short term impact of the organic inputs were both investigated by measuring VOCs emissions using a Proton Transfer Reaction-Mass Spectrometer (PTR-MS) and by analyzing active bacterial diversity by MiSeq Illumina sequencing just before and up to 64 days following the inputs. Soil VOCs emissions (diversity and fluxes) naturally varied with temperature and rainfall variations, irrespective of manure inputs. No effect of the 5-yr fertilization history was observed on bacterial communities’ composition and on soil VOCs emissions. However, both manure inputs (PS and MPS) were associated with an inoculation of γ-Proteobacteria (Pseudomonas sp. and/or Marinospirillum sp.) to the soil on top of which PS inputs activated native soil Bacillus sp. (Firmicutes). VOCs spectra was mainly dominated by methanol and acetonitrile, the acetonitrile emissions not depending on the organic practices. C-VOCs fluxes from the soil to the atmosphere varied from 12 to 76 μg of C-VOCs h−1 m−2 in the control plots. Pig slurry and methanized pig slurry differentially impacted soil VOCs emissions: PS inputs doubled the C-VOC fluxes due to high emission of methanol while MPS inputs reduced VOCs fluxes even less than the control unamended plots, which is of great interest in the context of mitigating greenhouse gases in agriculture. Our results suggest that soil fluxes could, under certain conditions, not be marginal compared to plant fluxes and be potentially driven by new land-uses in agriculture.
Auteurs, date et publication :
Auteurs Kevin Potard , Cécile Monard , Jean-Luc Le Garrec , Jean-Pierre Caudal , Nathalie Le Bris , Françoise Binet
Publication : Agriculture, Ecosystems & Environment
Date : 2025
Volume : 250
Pages : 25-36
Catégorie(s)
#INRAE #PRO #PRO EFELERésumé
The recycling of exogenous organic matter (EOM) through agriculture is an efficient way to enhance soil organic matter (SOM) and to supply crops with readily available nutrients. It can also cause environmental damage, such as nitrate leaching. Characterisation of EOM to predict the C and N dynamics of mineralisation when applied to cropped soils is essential to improve its use in agriculture. The measurement of C and N mineralisation through soil laboratory incubation of 18 types of EOM and EOM biochemical fractionation were used to parameterise the NCSOIL model to simulate the mineralisation kinetics of C and N. The soil type did not significantly interfere with EOM mineralisation and parameterisation, enabling extrapolation of the parameters for one soil to other soil types. Four groups of EOM were distinguished based on their C and N dynamics: (1) stable compost, (2) more reactive compost and stable manure, (3) manure and (4) very reactive EOM as sludge and litter that should be used as fertiliser. The use of easily accessible indicators, such as IROC (Lashermes et al., 2009) and the measured organic C:N ratio (CNEOM), was appropriate for parameterising groups 1 and 4. Regression relating the optimised resistant pool size and Van Soest fractions and CNEOM was found (R2 of 0.967) to improve the pool sizes for the remaining EOM. Further research is required to improve the parameterisation.
Auteurs, date et publication :
Auteurs P.E. Noirot-Cosson , K. Dhaouadi , V. Etievant , E. Vaudour , S. Houot
Publication : Soil Biology and Biochemistry
Date : 2025
Volume : 104
Pages : 128-140
Catégorie(s)
#INRAE #PRO #PRO QualiAgroAuteurs, date et publication :
Auteurs M. Chalhoub , Y. Coquet , P. Vachier
Publication : Vadose Zone Journal
Date : 2025
Volume : 12
Issue : 1
Pages : 11 p.
Catégorie(s)
#INRAE #PRO #PRO QualiAgroRésumé
Abstract. The implementation of agroecological practices often leads to additional soil organic carbon storage, and we have sought to assess the biogeochemical stability of this additional carbon. To achieve this, we implemented a multi-method approach using particle size and density fractionation, Rock-Eval® (RE) thermal analyses and long-term incubation (484 d), which we applied to topsoil samples (0–30 cm) from temperate Luvisols that had been subjected in >20-year-long experiments in France to conservation agriculture (CA), organic agriculture (ORG) and conventional agriculture (CON-LC) in the La Cage experiment and to organic waste product (OWP) applications in the QualiAgro experiment, including biowaste compost (BIOW), residual municipal solid waste compost (MSW), farmyard manure (FYM) and conventional agriculture without organic inputs (CON-QA). The additional carbon resulting from agroecological practices is the difference between the carbon stock of the bulk soil and physical fractions or carbon pools in the soil affected by agroecological practices and that of the same soil affected by a conventional practice used as control. The incubations provided information on the additional carbon stability in the short term (i.e. mean residence time, MRT, of <2 years) and showed that the additional soil organic carbon mineralized faster than the carbon in the conventional control at La Cage but slower at QualiAgro. In OWP-treated plots at QualiAgro, 60 %–66 % of the additional carbon was stored as mineral-associated organic matter (MAOM-C) and 34 %–40 % as particulate organic matter (POM-C). In CA and ORG systems at La Cage, 77 %–84 % of the additional carbon was stored as MAOM-C, whereas 16 %–23 % was stored as POM-C. Management practices hence influenced the distribution of additional carbon in physical fractions. Utilizing the PARTYSOC model with Rock-Eval® thermal analysis parameters, we found that most, if not all, of the additional carbon belonged to the active carbon pool (MRT∼30–40 years). In summary, our comprehensive multi-method evaluation indicates that the additional soil organic carbon is less stable over decadal and pluri-decadal timescales compared to soil carbon under conventional control conditions. Our results show that particle size and density fractions can be heterogenous in their biogeochemical stability. On the other hand, although the additional carbon is mainly associated with MAOM, the high proportion of this carbon in the active pool suggests that it has a mean residence time which does not exceed ∼50 years. Furthermore, agroecological practices with equivalent additional carbon stocks (MSW, FYM and CA) exhibited a higher proportion of additional carbon in POM-C under MSW (40 %) and FYM (34 %) compared to CA (16 %), which suggests a high chemical recalcitrance of POM-C under OWP management relative to conservation agriculture. Additional soil organic carbon derived from organic waste, i.e. biomass that has partially decomposed and has been transformed through its processing prior to its incorporation in soil, would be more biogeochemically stable in soil than that derived directly from plant biomass. The apparent contradictions observed between methods can be explained by the fact that they address different kinetic pools of organic carbon. Care must be taken to specify which range of residence times is considered when using any method with the intent to evaluate the biogeochemical stability of soil organic matter, as well as when using the terms stable or labile. In conclusion, the contrasting biogeochemical stabilities observed in the different management options highlight the need to maintain agroecological practices to keep these carbon stocks at a high level over time, given that the additional carbon is stable on a pluri-decadal scale.
Auteurs, date et publication :
Auteurs Tchodjowiè P. I. Kpemoua , Pierre Barré , Sabine Houot , François Baudin , Cédric Plessis , Claire Chenu
Publication : SOIL
Date : 2024
Volume : 10
Issue : 2
Pages : 533-549
Catégorie(s)
#INRAE #PRO #PRO QualiAgroAuteurs, date et publication :
Auteurs N. Colbach , C. Tschudy , D. Meunier , S. Houot , B. Nicolardot
Publication : European Journal of Agronomy
Date : 2025
Volume : 45
Pages : 7-19
Catégorie(s)
#INRAE #PRO #PRO QualiAgroRésumé
Generic biomarkers are needed to assess environmental risks in metal polluted soils. We assessed the strength of the relationship between earthworm energy reserves and metal availability under conditions of cocktail of metals at low doses and large range of soil parameters. Aporrectodea caliginosa was exposed in laboratory to a panel of soils differing in Cd, Pb and Zn total and available (CaCl2 and EDTA-extractable) concentrations, and in soil texture, pH, CEC and organic-C. Glycogen, protein and lipid contents were recorded in exposed worms. Glycogen contents were not linked to the explaining variables considered. Variable selection identified CaCl2 extractable metals concentrations and soil texture as the main factors affecting protein and lipid contents. The results showed opposite effects of Pb and Zn, high interindividual variability of biomarkers and weak relationships with easily extractable metals. Our results support the lack of genericity of energy reserves in earthworms exposed to field-contaminated soils.
Auteurs, date et publication :
Auteurs Léa Beaumelle , Isabelle Lamy , Nathalie Cheviron , Mickaël Hedde
Publication : Environmental Pollution
Date : 2025
Pages : 8
Catégorie(s)
#BiochemEnv #INRAE #PRO #PRO QualiAgroAuteurs, date et publication :
Auteurs M. Tella , E. Doelsch , P. Letourmy , S. Chataing , F. Cuoq , M. N. Bravin , H. Saint Macary
Publication : Waste Management
Date : 2025
Volume : 33
Issue : 1
Pages : 184-92