Résumé
Using organic waste products (OWP) in agriculture has been reported to impact both the activity and composition of soil microbial communities. However, little information is available on how the response of a soil microbial community to a given OWP may depend on the physicochemical and microbial properties of the soil receiving the input. Here, we performed a microcosm experiment to compare the effect of 2 different OWPs (GWS: co-compost of Green Wastes and Sewage sludge or FYM: FarmYard Manure), each applied to 5 different soils, on the activity, abundance and diversity of the soil microbial communities. Soils were selected to represent a range of physicochemical and climatic characteristics. CO2 and N2O emissions, microbial biomass and taxonomic diversity were monitored for 28 days following OWP input. The five soils presented different prokaryotic and fungal communities structures before OWP application. During the 28 days of incubation, those control soils (without OWP) harboring the highest organic matter contents released the greatest CO2 and N2O emissions, and had the highest soil microbial biomass. The impact of organic amendments on soil activity and microbial diversity differed with the nature of the OWP. FYM application increased CO2 emissions 2-fold and delayed N2O emissions compared to GWS. Major changes in prokaryotic genetic structures were also observed when GWS was applied. The effect of OWPs was dependent on soil type and the five soils exhibited distinct patterns of CO2 and N2O emission after a given input. This accorded with the fact that the structure and composition of the microbial communities harbored by each soil type responded differently to a given OWP application. To conclude, our results show that different soil types, harboring distinct microbial community structures, responded differently to OWP application, leading to different patterns and rates of greenhouse gas emissions. This response was also OWP-dependent.
Auteurs, date et publication :
Auteurs Sophie Sadet-Bourgeteau , Sabine Houot , Battle Karimi , Olivier Mathieu , Vincent Mercier , Denis Montenach , Thierry Morvan , Valerie Sappin-Didier , Francoise Watteau , Virginie Nowak , Samuel Dequiedt , Pierre-Alain Moron
Publication : APPLIED SOIL ECOLOGY
Date : 2019
Volume : 143
Pages : 70-79
Catégorie(s)
#ANR-Citation #INRAE #PRO #PRO ColmarRésumé
The occurrence of Pseudomonas aeruginosa was monitored at a broad spatial scale in French agricultural soils, from various soil types and under various land uses to evaluate the ability of soil to be a natural habitat for that species. To appreciate the impact of agricultural practices on the potential dispersion of P. aeruginosa, we further investigated the impact of organic amendment at experimental sites in France and Burkina Faso. A real-time quantitative PCR (qPCR) approach was used to analyze a set of 380 samples selected within the French RMQS (“Réseau de Mesures de la Qualité des Sols”) soil library. In parallel, a culture-dependent approach was tested on a subset of samples. The results showed that P. aeruginosa was very rarely detected suggesting a sporadic presence of this bacterium in soils from France and Burkina Faso, whatever the structural and physico-chemical characteristics or climate. When we analyzed the impact of organic amendment on the prevalence of P. aeruginosa, we found that even if it was detectable in various manures (at levels from 103 to 105 CFU or DNA targets (g drywt)−1 of sample), it was hardly ever detected in the corresponding soils, which raises questions about its survival. The only case reports were from a vineyard soil amended with a compost of mushroom manure in Burgundy, and a few samples from two fields amended with raw urban wastes in the sub-urban area of Ouagadougou, Burkina Faso. In these soils the levels of culturable cells were below 10 CFU (g drywt)−1.
Auteurs, date et publication :
Auteurs Amélie Deredjian , Céline Colinon , Edmond Hien , Elisabeth Brothier , Benjamin Youenou , Benoit Cournoyer , Samuel Dequiedt , Alain Hartmann , Claudy Jolivet , Sabine Houot , Lionel Ranjard , Nicolas P. A. Saby , Sylvie Nazaret
Publication : Frontiers in Cellular and Infection Microbiology
Date : 2014
Volume : 4
Catégorie(s)
#Genosol #INRAE #PRO #PRO ColmarRésumé
The purpose of this methodological study was to test whether similar soil hydraulic and solute transport properties could be estimated from field plots and lysimeter measurements. The transport of water and bromide (as an inert conservative solute tracer) in three bare field plots and in six bare soil lysimeters were compared. Daily readings of matric head and volumetric water content in the lysimeters showed a profile that was increasingly humid with depth. The hydrodynamic parameters optimized with HYDRUS-1D provided an accurate description of the experimental data for both the field plots and the lysimeters. However, bromide transport in the lysimeters was influenced by preferential transport, which required the use of the mobile/immobile water (MIM) model to suitably describe the experimental data. Water and solute transport observed in the field plots was not accurately described when using parameters optimized with lysimeter data (cross-simulation), and vice versa. The soil's return to atmospheric pressure at the bottom of the lysimeter and differences in tillage practices between the two set-ups had a strong impact on soil water dynamics. The preferential flow of bromide observed in the lysimeters prevented an accurate simulation of solute transport in field plots using the mean optimized parameters on lysimeters and vice versa.
Auteurs, date et publication :
Auteurs Arnaud Isch , Denis Montenach , Frederic Hammel , Philippe Ackerer , Yves Coquet
Publication : WATER
Date : 2019
Volume : 11
Issue : 6
Catégorie(s)
#ANR-Citation #INRAE #PRO #PRO ColmarAuteurs, date et publication :
Auteurs O. Sauzet , C. Cammas , P. Barbillon , M.P. Étienne , D. Montagne
Publication : Geoderma
Date : 2025
Volume : 266
Pages : 46-57
Catégorie(s)
#INRAE #PRO #PRO ColmarAuteurs, date et publication :
Auteurs T. Sterckeman , L. Gossiaux , S. Guimont , C. Sirguey , Z. Lin
Publication : Science of the Total Environment
Date : 2025
Volume : 639
Pages : 1440-1452
Catégorie(s)
#ANR-Citation #INRAE #PRO #PRO ColmarAuteurs, date et publication :
Auteurs F. Watteau , G. Villemin , F. Bartoli , C. Schwartz , J. L. Morel
Publication : Soil Biology and Biochemistry
Date : 2025
Volume : 46
Pages : 103-114