Auteurs, date et publication :
Auteurs Charlotte Salmon
Publication : La France Agricole
Date : 2022
Volume : 49
Catégorie(s)
#INRAE #PRORésumé
Environmental DNA (eDNA) metabarcoding is revolutionizing the monitoring of aquatic biodiversity. The use of eDNA has the potential to enable non-invasive, cost-effective, time-efficient and high-sensitivity monitoring of fish assemblages. Although the capacity of eDNA metabarcoding to describe fish assemblages is recognised, research efforts are still needed to better assess the spatial and temporal variability of the eDNA signal and to ultimately design an optimal sampling strategy for eDNA monitoring. In this context, we sampled three different lakes (a dam reservoir, a shallow eutrophic lake and a deep oligotrophic lake) every 6 weeks for 1 year. We performed four types of sampling for each lake (integrative sampling of sub-surface water along transects on the left shore, the right shore and above the deepest zone, and point sampling in deeper layers near the lake bottom) to explore the spatial variability of the eDNA signal at the lake scale over a period of 1 year. A metabarcoding approach was applied to analyse the 92 eDNA samples in order to obtain fish species inventories which were compared with traditional fish monitoring methods (standardized gillnet samplings). Several species known to be present in these lakes were only detected by eDNA, confirming the higher sensitivity of this technique in comparison with gillnetting. The eDNA signal varied spatially, with shoreline samples being richer in species than the other samples. Furthermore, deep-water samplings appeared to be non-relevant for regularly mixed lakes, where the eDNA signal was homogeneously distributed. These results also demonstrate a clear temporal variability of the eDNA signal that seems to be related to species phenology, with most of the species detected in spring during the spawning period on shores, but also a peak of detection in winter for salmonid and coregonid species during their reproduction period. These results contribute to our understanding of the spatio-temporal distribution of eDNA in lakes and allow us to provide methodological recommendations regarding where and when to sample eDNA for fish monitoring in lakes.
Auteurs, date et publication :
Auteurs Alix Hervé , Isabelle Domaizon , Jean-Marc Baudoin , Tony Dejean , Pierre Gibert , Pauline Jean , Tiphaine Peroux , Jean-Claude Raymond , Alice Valentini , Marine Vautier , Maxime Logez , Hideyuki Doi
Publication : PLOS ONE
Date : 2022
Volume : 17
Issue : 8
Pages : e0272660
Catégorie(s)
#INRAE #OLAAuteurs, date et publication :
Auteurs Emilie Réalis-Doyelle , Nathalie Cottin , Martin Daufresne , Emmanuel Naffrechoux , Stéphane Reynaud , Jean Guillard
Publication : Aquatic Toxicology
Date : 2025
Volume : 255
Pages : 106396
Catégorie(s)
#INRAE #OLAAuteurs, date et publication :
Auteurs Sonja Vospernik , Michael Heym , Hans Pretzsch , Maciej Pach , Mathias Steckel , Jorge Aldea , Gediminas Brazaitis , Andrés Bravo-Oviedo , Miren Del Rio , Magnus Löf , Marta Pardos , Kamil Bielak , Felipe Bravo , Lluís Coll , Jakub Černý , Lars Droessler , Martin Ehbrecht , Aris Jansons , Nathalie Korboulewsky , Marion Jourdan
Publication : Forest Ecology and Management
Date : 2025
Volume : 530
Pages : 120753
Catégorie(s)
#FORET OPTMix #INRAEAuteurs, date et publication :
Auteurs Nolwenn Decanter , Romane Normand , Ahmed Souissi , Catherine Labbé , Eric Edeline , Guillaume Evanno
Publication : Ecology and Evolution
Date : 2025
Volume : 13
Issue : 4
Pages : e9970
Catégorie(s)
#INRAE #PEARLRésumé
Abstract
River–lake transition zones have been identified as major drivers of phytoplankton growth. With climate change reducing the frequency of complete lake overturns, it is expected that the Rhône River, the main tributary to Lake Geneva (France/Switzerland), will become the major source of nutrients for the lake euphotic zone. The river–lake transition zone was hence examined at the mouth of the Rhône River with the aim of understanding the complexities and controls of phytoplankton distribution in this specific deltaic ecosystem. Two field campaigns were carried out in which water samples were collected from longitudinal and transversal transects across the transition zone. These samples were analyzed for both nutrient and phytoplankton concentrations, while the fraction of Rhône River water in a lake sample was determined by the stable isotope composition of the water. The results indicate contributions in P and Si related to the Rhône intrusion into the lake. Furthermore, this river–lake transition zone appears to be a dynamic area that can locally present optimal conditions for phytoplankton growth. In early spring, a wind event broke the early and weak stratification of the lake, forcing the Rhône River and its turbidity plume to intrude deeper. Thus, this sharp drop of the turbidity within the euphotic zone allowed an increase in the phytoplankton biovolume of 44%. In early fall, outside of the turbid near field of the river mouth, the Rhône interflow, located just below the thermocline, promoted a local deep chlorophyll maximum.
Auteurs, date et publication :
Auteurs Gabriel Cotte , Frédéric Soulignac , Fabio Dos Santos Correia , Matthieu Fallet , Bastiaan Willem Ibelings , David Andrew Barry , Torsten W. Vennemann
Publication : Aquatic Sciences
Date : 2025
Volume : 85
Issue : 2
Pages : 37
Catégorie(s)
#INRAE #OLAAuteurs, date et publication :
Auteurs Anaïs Thomas , Pierrick Priault , Séverine Piutti , Erwin Dallé , Nicolas Marron
Publication : Agroforestry Systems
Date : 2025
Volume : 97
Issue : 4
Pages : 673-686
Catégorie(s)
#FORET AgroTCR #INRAERésumé
In hardwater lakes, calcite precipitation is an important yet poorly understood process in the lacustrine carbon cycle, in which catchment-derived alkalinity (Alk) is both transformed and translocated. While the physico-chemical conditions supporting the supersaturation of water with respect to calcite are theoretically well described, the magnitude and conditions underlying calcite precipitation at fine temporal and spatial scales are poorly constrained. In this study, we used high frequency, depth-resolved (0–30 m) data collected over 18 months (June 2019 – November 2020) in the deeper basin of Lake Geneva to describe the dynamics of calcite precipitation fluxes at a fine temporal resolution (day to season) and to scale them to carbon fixation by primary production. Calcite precipitation occurred during the warm stratified periods when surface water CO2 concentrations were below atmospheric equilibrium. Seasonally, the extent of Alk loss due to calcite precipitation (i.e., [30–42] g C m−2) depended upon the level of Alk in surface waters. Moreover, interannual variability in seasonal calcite precipitation depended on the duration of stratification, which determined the volume of the water layer susceptible to calcite precipitation. At finer timescales, calcite precipitation was characterized by marked daily variability with dynamics strongly related to that of planktonic autotrophic metabolism. Increasing daily calcite precipitation rates (i.e., maximum values 9 mmol C m−3 d−1) coincided with increasing net ecosystem production (NEP) during periods of enhanced water column stability. In these conditions, calcite precipitation could remove as much inorganic carbon from the productive layers as NEP. This study provides mechanistic insights into the conditions driving pelagic calcite precipitation, and quantifies its essential contribution to the coupling of organic and inorganic carbon cycling in lakes.
Auteurs, date et publication :
Auteurs Nicolas Escoffier , Pascal Perolo , Gaël Many , Natacha Tofield Pasche , Marie-Elodie Perga
Publication : Science of The Total Environment
Date : 2025
Volume : 864
Pages : 160699
Catégorie(s)
#INRAE #OLAAuteurs, date et publication :
Auteurs P Belleville , F Keuper , F Bornet , J Duval , F Ferchaud , E Gréhan , B Mary , G Vitte , B Heinesch , B Dumont , J Léonard
Date : 2025
Catégorie(s)
#ACBB #ACBB Mons #INRAEAuteurs, date et publication :
Auteurs R Calone , A Fiore , ML Cayuela , A Lagomarsino , J Léonard , F Ferchaud
Date : 2025