Auteurs, date et publication :
Auteurs L. Jacquin , Q. Mori , V. MéDoc
Publication : Parasitology
Date : 2025
Volume : 140
Issue : 10
Pages : 1310-1315
Catégorie(s)
#CNRS #ENS #PLANAQUARésumé
Highly differentiated mature spermatozoa carry not only genetic but also epigenetic information that is to be transmitted to the embryo. DNA methylation is one epigenetic actor associated with sperm nucleus compaction, gene silencing, and prepatterning of embryonic gene expression. Therefore, the stability of this mark toward reproductive biotechnologies is a major issue in animal production. The present work explored the impact of hormonal induction of spermiation and sperm cryopreservation in two cyprinids, the goldfish (Carassius auratus) and the zebrafish (Danio rerio), using LUminometric Methylation Assay (LUMA). We showed that while goldfish hormonal treatment did increase sperm production, it did not alter global DNA methylation of spermatozoa. Different sperm samples repeatedly collected from the same males for 2 months also showed the same global DNA methylation level. Similarly, global DNA methylation was not affected after cryopreservation of goldfish spermatozoa with methanol, whereas less efficient cryoprotectants (dimethylsulfoxide and 1,2-propanediol) decreased DNA methylation. In contrast, cryopreservation of zebrafish spermatozoa with methanol induced a slight, but significant, increase in global DNA methylation. In the less compact nuclei, that is, goldfish fin somatic cells, cryopreservation did not change global DNA methylation regardless of the choice of cryoprotectant. To conclude, global DNA methylation is a robust parameter with respect to biotechnologies such as hormonal induction of spermiation and sperm cryopreservation, but it can be altered when the best sperm manipulation conditions are not met.
Auteurs, date et publication :
Auteurs Alexandra Depincé , Anne Gabory , Katarzyna Dziewulska , Pierre-Yves Le Bail , Hélène Jammes , Catherine Labbé
Publication : Molecular Reproduction and Development
Date : 2025
Volume : 87
Issue : 1
Pages : 124-134
Catégorie(s)
#CNRS #EcoGenO #Université de RennesAuteurs, date et publication :
Auteurs Elodie A Courtois , CE Timothy Paine , Pierre-Alain Blandinieres , Didier Stien , Jean-Marie Bessiere , Emeline Houel , Christopher Baraloto , Jerome Chave
Publication : Journal of chemical ecology
Date : 2025
Volume : 35
Issue : 11
Pages : 1349-1362
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs DC Lees , AY Kawahara , R Rougerie , I Ohshima , A Kawakita , O Bouteleux , J De Prins , C Lopez‐Vaamonde
Publication : Molecular Ecology Resources
Date : 2025
Volume : 14
Issue : 2
Pages : 286-296
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs Thibaud Decaëns , David Porco , Samuel W James , George G Brown , Vincent Chassany , Florence Dubs , Lise Dupont , Emmanuel Lapied , Rodolphe Rougerie , Jean-Pierre Rossi
Publication : Soil Biology and Biochemistry
Date : 2025
Volume : 92
Pages : 171-183
Catégorie(s)
#CNRS #FORET NouraguesRésumé
What are the long-term consequences of invasive species? After invasion, how long do ecosystems require to reach a new equilibrium? Answering these questions requires long-term, high-resolution data that are vanishingly rare. We combined the analysis of environmental DNA extracted from a lake sediment core, coprophilous fungi, and sedimentological analyses to reconstruct 600 years of ecosystem dynamics on a sub-Antarctic island and to identify the impact of invasive rabbits. Plant communities remained stable from AD 1400 until the 1940s, when the DNA of invasive rabbits was detected in sediments. Rabbit detection corresponded to abrupt changes of plant communities, with a continuous decline of a dominant plant species. Furthermore, erosion rate abruptly increased with rabbit abundance. Rabbit impacts were very fast and were stronger than the effects of climate change during the 20th century. Lake sediments can allow an integrated temporal analysis of ecosystems, revealing the impact of invasive species over time and improving our understanding of underlying mechanisms.
Auteurs, date et publication :
Auteurs Gentile Francesco Ficetola , Jérôme Poulenard , Pierre Sabatier , Erwan Messager , Ludovic Gielly , Anouk Leloup , David Etienne , Jostein Bakke , Emmanuel Malet , Bernard Fanget , Eivind Støren , Jean-Louis Reyss , Pierre Taberlet , Fabien Arnaud
Publication : Science Advances
Date : 2018
Volume : 4
Issue : 5
Pages : eaar4292
Catégorie(s)
#CNRS #eDNAAuteurs, date et publication :
Auteurs NG Yoccoz , KA Bråthen , L Gielly , J Haile , ME Edwards , T Goslar , H Von Stedingk , AK Brysting , E Coissac , F Pompanon
Publication : Molecular Ecology
Date : 2025
Volume : 21
Issue : 15
Pages : 3647-3655
Catégorie(s)
#CNRS #FORET NouraguesRésumé
Plants are widely recognized as chemical factories, with each species producing dozens to hundreds of unique secondary metabolites. These compounds shape the interactions between plants and their natural enemies. Here we explore how plants generate chemical diversity, and what evolutionary processes have led to novel compounds and unique chemical profiles.We comprehensively characterized the chemical profile of one-third of the species of tropical rainforest trees in the genus Inga (∼ 100, Fabaceae) and applied phylogenetic comparative methods to understand the mode of chemical defense evolution.We show that: 1) Each Inga species produces exceptionally high levels of phytochemical diversity, despite costs, tradeoffs and biosynthetic constraints. 2) Closely related species have highly divergent defense profiles, with individual compounds, major compound classes and complete profiles showing little to no phylogenetic signal. 3) We show that the evolution of a species’ chemical profile shows a signature of divergent adaptation, implying that it is advantageous for a species to have distinct chemistry from close relatives to avoid shared natural enemies. 4) Finally, we hypothesize a model where deep homology of biosynthetic pathways and rapid changes in regulatory mechanisms may better explain the observed large shifts in defense chemicals between closely related taxa.Competing Interest StatementThe authors have declared no competing interest.
Auteurs, date et publication :
Auteurs Dale L. Forrister , María-José Endara , Abrianna J. Soule , Gordon C. Younkin , Anthony G. Mills , John Lokvam , Kyle G. Dexter , R. Toby Pennington , Catherine A. Kidner , James A. Nicholls , Oriane Loiseau , Thomas A. Kursar , Phyllis D. Coley
Publication : bioRxiv
Date : 2021
Pages : 2021.12.17.473194
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs Marisa De Campos-Santana , Gerardo Robledo , Cony Decock , Rosa Mara Borges Da Silveira
Publication : Cryptogamie, Mycologie
Date : 2025
Volume : 36
Issue : 1
Pages : 43-78
Catégorie(s)
#CNRS #FORET NouraguesAuteurs, date et publication :
Auteurs Julio V Schneider , Juraj Paule , Jailson Gitaí , Stefan Dressler , Cássia Lima Silva Gusmão , Ana Maria Benko-Iseppon
Publication : Botanical journal of the Linnean Society
Date : 2025
Volume : 177
Issue : 1
Pages : 1-14