Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA

Résumé

1. Multi-taxa diversity survey was done from DNA contained in 10 g soil samples.
2. DNA extraction protocols focusing on extracellular or total DNA were compared.
3. Few differences were observed between the two protocols.
4. The extracellular DNA extraction protocol was more time/cost effective.
5. It is appropriate for broad taxonomic and large-scale DNA-based surveys.


Auteurs, date et publication :

Auteurs Lucie Zinger , Jérôme Chave , Eric Coissac , Amaia Iribar , Eliane Louisanna , Sophie Manzi , Vincent Schilling , Heidy Schimann , Guilhem Sommeria-Klein , Pierre Taberlet

Publication : Soil Biology and Biochemistry

Date : 2025

Volume : 96

Pages : 16-19


Catégorie(s)

#ANR-Citation #CNRS #FORET Nouragues