Auteurs, date et publication :
Auteurs Carlos Barata , Bruno Campos , Claudia Rivetti , Gerald A. LeBlanc , Stephanie Eytcheson , Stephanie McKnight , Marysia Tobor-Kaplon , Selinda de Vries Buitenweg , Suhyon Choi , Jinhee Choi , Elena I Sarapultseva , Marie-Agnès Coutellec , Maïra Coke , Pascal Pandard , Arnaud Chaumot , Hervé Quéau , Nicolas Delorme , Olivier Geffard , Fernando Martínez-Jerónimo , Haruna Watanabe
Publication : Science of The Total Environment
Date : 2017
Volume : 579
Pages : 1073-1083
Catégorie(s)
#INRAE #PEARLAuteurs, date et publication :
Auteurs Gaétan Pottier
Date : 2017
Catégorie(s)
#INRAE #PEARLAuteurs, date et publication :
Auteurs Emeline Chesneau , Gabrielle Thiébaut , Jacques Haury
Date : 2025
Catégorie(s)
#INRAE #PEARLRésumé
The data were collected from experimental freshwater fishpond systems (extensive, semi-intensive and semi-intensive coupled with lagoon). The dataset presented is composed of:
- Individual Fish parameters (live weight, length at fork) at stocking and harvest for the three species reared: common carp (Cyprinus carpio), roach (Rutilus rutilus) and tench (Tinca tinca).
- Physical indicators of the water quality in pond, monthly measured :temperature, pH, conductivity, dissolved oxygen, oxygen saturation and water transparency
- Physical indicators of the water quality in pond, measured every 5 minutes on 24h in September 2014: temperature, pH, oxydo-reduction potential, conductivity, dissolved oxygen and oxygen saturation.
- Concentration in pond water of chemical compounds , monthly sampled :total nitrogen (TN), ammonium, nitrite, nitrate, orthophosphate and total phosphorus(TP)
- Concentration in pond water of chemical compounds, sampled 5 minutes on 24h in September 2014: TN, ammonium, nitrate, orthophosphate, TP
- Concentration of chlorophyll in pond water monthly sampled: chlorophyll-a from March to June and total chlorophyll, blue chlorophyll, green chlorophyll and brown chlorophyll from July to November.
- Concentration in TN and TP in the sediment of each pond at fish stocking and harvest.
Auteurs, date et publication :
Auteurs Christophe Jaeger , Joël Aubin
Date : 2025
Catégorie(s)
#INRAE #PEARLRésumé
Natural products have proven to be an immeasurable source of bioactive compounds. The exceptional biodiversity encountered in Amazonia, alongside a rich entomofauna and frequent interactions with various herbivores is the crucible of a promising chemodiversity. This prompted us to search for novel botanical insecticides in French Guiana. As this French overseas department faces severe issues linked to insects, notably the strong incidence of vector-borne infectious diseases, we decided to focus our research on products able to control the mosquito Aedes aegypti. We tested 452 extracts obtained from 85 species originating from 36 botanical families and collected in contrasted environments against an Ae. aegypti laboratory strain susceptible to all insecticides, and a natural population resistant to both pyrethroid and organophosphate insecticides collected in Cayenne for the most active of them. Eight species (Maytenus oblongata Reissek, Celastraceae; Costus erythrothyrsus Loes., Costaceae; Humiria balsamifera Aubl., Humiriaceae; Sextonia rubra (Mez) van der Werff, Lauraceae; Piper hispidum Sw., Piperaceae; Laetia procera (Poepp.) Eichl., Salicaceae; Matayba arborescens (Aubl.) Radlk., Sapindaceae; and Cupania scrobitulata Rich., Sapindaceae) led to extracts exhibiting more than 50% larval mortality after 48 h of exposition at 100 µg/mL against the natural population and were considered active. Selectivity and phytochemistry of these extracts were therefore investigated and discussed, and some active compounds highlighted. Multivariate analysis highlighted that solvents, plant tissues, plant family and location had a significant effect on mortality while light, available resources and vegetation type did not. Through this case study we highlighted that plant defensive chemistry mechanisms are crucial while searching for novel insecticidal products.
Auteurs, date et publication :
Auteurs Michaël Falkowski , Arnaud Jahn-Oyac , Guillaume Odonne , Claudiane Flora , Yannick Estevez , Seindé Touré , Isabelle Boulogne , Jean-Charles Robinson , Didier Béreau , Philippe Petit , Didier Azam , Maïra Coke , Jean Issaly , Pascal Gaborit , Didier Stien , Véronique Eparvier , Isabelle Dusfour , Emeline Houël
Publication : Acta Tropica
Date : 2025
Volume : 201
Pages : 105179
Catégorie(s)
#ANR-Citation #INRAE #PEARLRésumé
Background: Goldfish is an important model for various areas of research, including neural development and behavior and a species of significant importance in aquaculture, especially as an ornamental species. It has a male heterogametic (XX/XY) sex determination system that relies on both genetic and environmental factors, with high temperatures being able to produce female-to-male sex reversal. Little, however, is currently known on the molecular basis of genetic sex determination in this important cyprinid model. Here we used sequencing approaches to better characterize sex determination and sex-chromosomes in an experimental strain of goldfish. Results: Our results confirmed that sex determination in goldfish is a mix of environmental and genetic factors and that its sex determination system is male heterogametic (XX/XY). Using reduced representation (RAD-seq) and whole genome (pool-seq) approaches, we characterized sex-linked polymorphisms and developed male specific genetic markers. These male specific markers were used to distinguish sex-reversed XX neomales from XY males and to demonstrate that XX female-to-male sex reversal could even occur at a relatively low rearing temperature (18 degrees C), for which sex reversal has been previously shown to be close to zero. We also characterized a relatively large non-recombining region (similar to 11.7 Mb) on goldfish linkage group 22 (LG22) that contained a high-density of male-biased genetic polymorphisms. This large LG22 region harbors 373 genes, including a single candidate as a potential master sex gene, i.e., the anti-Mullerian hormone gene (amh). However, no sex-linked polymorphisms were detected in the coding DNA sequence of the goldfishamhgene. Conclusions: These results show that our goldfish strain has a relatively large sex locus on LG22, which is likely the Y chromosome of this experimental population. The presence of a few XX males even at low temperature also suggests that other environmental factors in addition to temperature could trigger female-to-male sex reversal. Finally, we also developed sex-linked genetic markers, which will be important tools for future research on sex determination in our experimental goldfish population. However, additional work would be needed to explore whether this sex locus is conserved in other populations of goldfish.
Auteurs, date et publication :
Auteurs Ming Wen , Romain Feron , Qiaowei Pan , Justine Guguin , Elodie Jouanno , Amaury Herpin , Christophe Klopp , Cedric Cabau , Margot Zahm , Hugues Parrinello , Laurent Journot , Shawn M. Burgess , Yoshihiro Omori , John H. Postlethwait , Manfred Schartl , Yann Guiguen
Publication : BMC GENOMICS
Date : 2020
Volume : 21
Issue : 1
Catégorie(s)
#ANR-Citation #INRAE #PEARLAuteurs, date et publication :
Auteurs Luis Portillo Lemus , Michel Bozec , Marilyne Harang , Julie Coudreuse , Jacques Haury , Solenn Stoeckel , Dominique Barloy
Publication : Plant-Environment Interactions
Date : 2025
Volume : 2
Issue : 2
Pages : 74
Catégorie(s)
#INRAE #PEARLAuteurs, date et publication :
Auteurs Nolwenn Decanter , Romane Normand , Ahmed Souissi , Catherine Labbé , Eric Edeline , Guillaume Evanno
Publication : Ecology and Evolution
Date : 2025
Volume : 13
Issue : 4
Pages : e9970
Catégorie(s)
#INRAE #PEARLAuteurs, date et publication :
Auteurs Margot Wagner‐Deyriès , Léa Varignier , Marion Revel , Thomas Delhaye , David Rondeau , Marie‐Agnès Coutellec , R.J. Scott McCairns
Publication : Environmental Toxicology and Chemistry
Date : 2023
Pages : etc.5564
Catégorie(s)
#INRAE #PEARLRésumé
Sextonia rubra is an Amazonian tree known for the larvicide activity of its wood extracts against Aedes aegypti. However, the lipophilic character of this extract presents an obstacle to the development of vector management products. The purpose of this study was to assess the feasibility, efficacy, and selectivity of a water-soluble formulation based on a S. rubra wood extract characterized by its larvicidal compounds rubrenolide and rubrynolide. Using reference and wild strains, larval mortality, oviposition behavior, hatching response, and fitness of mosquitoes exposed to the extract were evaluated. Laboratory eco- and cytotoxicity bioassays were performed concurrently. The extract showed larvicidal efficacy and no repulsive effect on oviposition, thus supporting its use in the treatment of breeding sites. The formulation exhibited strong acute toxicity, leading to 100% larval mortality in 2–4 days in either the dry or rainy season at a test dose of 102 µg/ml under semi-operational conditions, with residual activity maintained during the 5 weeks of testing. The formulation allowed for a noticeable gain in selectivity compared with the extract, with lower immobilization rates for Daphnia magna and Chironomus riparius, and no significant toxicity towards human cell lines (IC50 > 100 µg/ml). These results highlight the potential of the S. rubra-based formulation for the management of Ae. aegypti larvae, including strains showing resistance to conventional insecticides.
Auteurs, date et publication :
Auteurs Emmanuelle Clervil , Jean-Bernard Duchemin , Nadine Amusant , Edmond Wozniak , Didier Azam , Maïra Coke , Dominique Huteau , Rémy Le Guével , Hélène Solhi , Isabelle Dusfour , Emeline Houël
Publication : Journal of Pest Science
Date : 2024